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peptide exchange assay buffer #2  (MBL International)
90
MBL International peptide exchange assay buffer #2
SARS-CoV-2 <t>peptide</t> <t>exchange</t> with QuickSwitch HLA-A*02:01 Tetramer-PE molecules. 50 µg/mL Tetramerized and R-phycoerythrin coupled HLA-A*02:01 molecules were used for the peptide exchange experiment. All HLA-A*02:01 molecules were loaded with a exiting peptide and incubated with 20 µM of competing SARS-CoV-2 peptides for 4 h at room temperature (≈ 23 °C) in the presence of a kit-determined volume of Peptide Exchange Factor #1 (PEF#1). The amount of exiting peptide removed from the MHC molecule was then determined following a capture <t>assay</t> and plotted on the vertical axis for each tested peptide.
Peptide Exchange Assay Buffer #2, supplied by MBL International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peptide exchange assay buffer #2 - by Bioz Stars, 2026-03
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SARS-CoV-2 peptide exchange with QuickSwitch HLA-A*02:01 Tetramer-PE molecules. 50 µg/mL Tetramerized and R-phycoerythrin coupled HLA-A*02:01 molecules were used for the peptide exchange experiment. All HLA-A*02:01 molecules were loaded with a exiting peptide and incubated with 20 µM of competing SARS-CoV-2 peptides for 4 h at room temperature (≈ 23 °C) in the presence of a kit-determined volume of Peptide Exchange Factor #1 (PEF#1). The amount of exiting peptide removed from the MHC molecule was then determined following a capture assay and plotted on the vertical axis for each tested peptide.

Journal: Vaccine

Article Title: Assessment of SARS-CoV-2 specific CD4(+) and CD8 (+) T cell responses using MHC class I and II tetramers

doi: 10.1016/j.vaccine.2021.03.008

Figure Lengend Snippet: SARS-CoV-2 peptide exchange with QuickSwitch HLA-A*02:01 Tetramer-PE molecules. 50 µg/mL Tetramerized and R-phycoerythrin coupled HLA-A*02:01 molecules were used for the peptide exchange experiment. All HLA-A*02:01 molecules were loaded with a exiting peptide and incubated with 20 µM of competing SARS-CoV-2 peptides for 4 h at room temperature (≈ 23 °C) in the presence of a kit-determined volume of Peptide Exchange Factor #1 (PEF#1). The amount of exiting peptide removed from the MHC molecule was then determined following a capture assay and plotted on the vertical axis for each tested peptide.

Article Snippet: Following a 150 μL wash with peptide exchange assay buffer #2 (MBLI: KXT-T07001 provided in the kit, must be diluted 1:10 before use) the exchanged MHC Class II molecules were stained with 25 μL of exiting peptide antibody-FITC (MBLI: KXT-T07201 provided in the kit).

Techniques: Incubation

SARS-CoV-2 peptide exchange with QuickSwitch HLA-DRB1*01:01, HLA-DRB1*04:01 and HLA-DRB1*15:01 molecules. 100 µg/mL recombinant HLA-DRB1*01:01 (Blue Bars), HLA-DRB1*04:01 (Orange Bars) and HLA-DRB1*15:01 (Gray Bars) molecules were used for the peptide exchange experiment. All MHC Class II molecules were loaded with a QuickSwitch exiting peptide and incubated with 1 mM of competing SARS-CoV-2 peptides overnight (greater than10 h) at 37 °C. The percentage of exiting peptide removed from the MHC molecule was then determined following a capture assay and plotted on the vertical axis for each tested peptide. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Vaccine

Article Title: Assessment of SARS-CoV-2 specific CD4(+) and CD8 (+) T cell responses using MHC class I and II tetramers

doi: 10.1016/j.vaccine.2021.03.008

Figure Lengend Snippet: SARS-CoV-2 peptide exchange with QuickSwitch HLA-DRB1*01:01, HLA-DRB1*04:01 and HLA-DRB1*15:01 molecules. 100 µg/mL recombinant HLA-DRB1*01:01 (Blue Bars), HLA-DRB1*04:01 (Orange Bars) and HLA-DRB1*15:01 (Gray Bars) molecules were used for the peptide exchange experiment. All MHC Class II molecules were loaded with a QuickSwitch exiting peptide and incubated with 1 mM of competing SARS-CoV-2 peptides overnight (greater than10 h) at 37 °C. The percentage of exiting peptide removed from the MHC molecule was then determined following a capture assay and plotted on the vertical axis for each tested peptide. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Following a 150 μL wash with peptide exchange assay buffer #2 (MBLI: KXT-T07001 provided in the kit, must be diluted 1:10 before use) the exchanged MHC Class II molecules were stained with 25 μL of exiting peptide antibody-FITC (MBLI: KXT-T07201 provided in the kit).

Techniques: Recombinant, Incubation